3-Methylcholanthrene treated Rat

ABSTRACT: Analysis of transcriptional response changes in rats after treatment with 3-Methylcholanthrene Rats were treated with 3-methylcholanthrene (MC) (100 umol/kg) in corn oil (CO) (2 ml/kg), or were given equal volumes of CO, i.p., once daily for 4 days. At 1, 15, and 28 day time points, the animals were sacrificed, and livers were stored at -800 C for later isolation of RNA. Gene expression was studied by cDNA microarray analyses. The analyses was conducted in the laboratory of Dr. Chris Bradfield of the University of Wisconsin. Gene expression was monitored using 4572 unique clones from liver-derived EST libraries fortified with clones of known liver genes representing ~4000 genes. These cDNAs represented toxicologically relevant genes such as drug metabolizing enzymes (e.g. P450s), infl

ORGANISM(S): Rattus norvegicus

SUBMITTER: Aaron Vollrath 

PROVIDER: E-GEOD-3175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress