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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles

ABSTRACT: In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and susceptible alleles at the wheat Fhb1 locus. The objectives of this study were: (1) to identify the overall response in wheat to F. graminearum infection; (2) to identify key genes involved in FHB resistance/susceptibility pathways in wheat; (3) to compare the transcript profiles of wheat and barley during F. graminearum infection; and (4) to examine the relationship between transcript accumulation, disease severity, fungal biomass and trichothecene accumulation in wheat. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is TA20 at PLEXdb.] FHB resistant and susceptible plants 48 an 96 hours after treatment with Fusarium graminearum strain Butte 86

ORGANISM(S): Triticum aestivum

SUBMITTER: Seungho Cho

PROVIDER: E-GEOD-31758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of a wheat near-isogenic line pair carrying Fusarium head blight-resistant and -susceptible alleles.

Jia Haiyan H Cho Seungho S Muehlbauer Gary J GJ

Molecular plant-microbe interactions : MPMI 20091101 11

Fusarium head blight (FHB), caused primarily by Fusarium graminearum, decreases grain yield and quality in wheat and barley. Disease severity, deoxynivalenol (DON), fungal biomass, and transcript accumulation were examined in a wheat near-isogenic line pair carrying either the resistant or susceptible allele for the chromosome 3BS FHB-resistance quantitative trait locus (Fhb1). Fhb1 restricts spread of disease symptoms but does not provide resistance to initial infection or initial DON accumulat ...[more]

PMID: 19810806

Similar Datasets

Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles

Project description:In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and susceptible alleles at the wheat Fhb1 locus. The objectives of this study were: (1) to identify the overall response in wheat to F. graminearum infection; (2) to identify key genes involved in FHB resistance/susceptibility pathways in wheat; (3) to compare the transcript profiles of wheat and barley during F. graminearum infection; and (4) to examine the relationship between transcript accumulation, disease severity, fungal biomass and trichothecene accumulation in wheat. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is TA20 at PLEXdb.]

2011-08-31 | GSE31758 | GEO

Barley cv Morex inoculated with Fusarium graminearum and water as mock control

Project description:Barley cv. Morex inoculated with Fusarium graminearum (isolate Butte 86) or water (mock). Sampled at 24, 48, 72, 96 and 144 hours after treatment. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jayanand Boddu. The equivalent experiment is BB9 at PLEXdb.] time: 24 - Treated or untreated: WATER(5-replications); time: 24 - Treated or untreated: Fusarium(5-replications); time: 48 - Treated or untreated: WATER(4-replications); time: 48 - Treated or untreated: Fusarium(4-replications); time: 72 - Treated or untreated: WATER(5-replications); time: 72 - Treated or untreated: Fusarium(5-replications); time: 96 - Treated or untreated: WATER(4-replications); time: 96 - Treated or untreated: Fusarium(4-replications); time: 144 - Treated or untreated: WATER(4-replications); time: 144 - Treated or untreated: Fusarium(4-replications)

2011-11-22 | E-GEOD-33407 | biostudies-arrayexpress

Transcriptome analysis of trichothecene-induced gene expression in barley

Project description:Fusarium Head Blight susceptible barley variety, Morex, was infected with deoxynivalenol production deficient mutant strain (GZT40) and wild type stains (Z3639) of Fusarium graminearum. The RNA was sampled at 48 and 96 hours after inoculation. and was used hybridize to Barley_1 GeneChip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jayanand Boddu. The equivalent experiment is BB52 at PLEXdb.] time: 48 hai. - Treated or untreated: WATER(3-replications); time: 48 hai. - Treated or untreated: MUTANT(3-replications); time: 48 hai. - Treated or untreated: WILD TYPE(3-replications); time: 96 hai. - Treated or untreated: WATER(3-replications); time: 96 hai. - Treated or untreated: MUTANT(3-replications); time: 96 hai. - Treated or untreated: WILD TYPE(3-replications)

2011-11-22 | E-GEOD-33398 | biostudies-arrayexpress

Transcription profiling of wheat development

Project description:The analysis of gene expression during wheat development:; Gene expression measurements were carried out on a developmental tissue; series for wild-type wheat (cv. Chinese Spring) using the Affymetrix; Wheat GeneChip. Thirteen tissues at defined developmental stages were; chosen to match the barley (cv. Morex) tissue series of Druka et al. 2006 that used the Affymetrix Barley1 GeneChip. Three replicates of:; root tissue at two different developmental stages, leaf, crown,; caryopsis, anther, pistil, inflorescence, bracts, mesocotyl, endosperm,; embryo and coleoptiles were hybridised. Comparisons between this wheat; data and the barley dataset were performed and are available at; http://contigcomp.acpfg.com.au ; [PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tim Sutton. The equivalent experiment is TA3 at PLEXdb.] Experiment Overall Design: tissue type: germinating seed, coleoptile(3-replications); tissue type: germinating seed, root(3-replications); tissue type: germinating seed, embryo(3-replications); tissue type: seedling, root(3-replications); tissue type: seedling, crown(3-replications); tissue type: seedling, leaf(3-replications); tissue type: immature inflorescence(3-replications); tissue type: floral bracts, before anthesis(3-replications); tissue type: pistil, before anthesis(3-replications); tissue type: anthers, before anthesis(3-replications); tissue type: 3-5 DAP caryopsis(3-replications); tissue type: 22 DAP embryo(3-replications); tissue type: 22 DAP endosperm(3-replications)

2009-08-15 | E-GEOD-12508 | biostudies-arrayexpress

Transcriptomic analysis of F. graminearum disease response in near-isogenic resistant and susceptible hexaploid wheat lines

Project description:Fusarium graminearum causes Fusarium head blight (FHB), which represents one of the major wheat diseases worldwide, determining reduction in grain quality, yield and the accumulation of mycotoxins. To mine the molecular response associated to the wheat 2DL FHB resistance quantitative trait locus (QTL), derived from the cultivar Wuhan-1, and to identify candidate genes implicated in such resistance, a comprehensive transcriptomic analysis of the early response to F. graminearum infection at 3 days post inoculation of spikelet and rachis was performed with the RNA-Seq and miRNA-Seq techniques. The analyses were conducted on two Near Isogenic Lines (NILs) differing for the presence of the 2DL QTL (2-2618, resistant 2DL+ and 2-2890, susceptible null).

2018-02-28 | E-MTAB-6383 | biostudies-arrayexpress

Microarray of barley genes in wheat-barley chromosome addition lines

Project description:Transcript levels of barley genes were examined in the wheat-barley chromosome addition lines having one of six barley chromomes, 2H, 3H, 4H, 5H, 6H and 7H. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is BB8 at PLEXdb.] genotype: chr2H(3-replications); genotype: chr2HL(3-replications); genotype: chr3H(3-replications); genotype: chr4H(3-replications); genotype: chr5H(3-replications); genotype: chr6H(3-replications); genotype: chr6HL(3-replications); genotype: chr6HS(3-replications); genotype: chr7H(3-replications); genotype: Betzes(3-replications); genotype: Chinese Spring(3-replications)

2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

Dual RNA-sequencing of Fusarium Head Blight resistance in winter wheat

Project description:In this study, we used dual RNA-sequencing to profile FHB-resistant AC Emerson, FHB-moderately AC Morley, and FHB-susceptible CDC Falcon winter wheat cultivars prior to and in response to Fusarium graminearum at 7 days post inoculation. Differential expression analyses revealed distinct defense responses between resistant and susceptible wheat cultivars including increased mechanical defense through lignin biosynthesis and increased deoxynivalenol (DON) detoxification through UDP-glycosyltransferase activity in resistant cultivars. Further, differential expression analysis in F. graminearum challenging these distinct cultivars revealed changes genes involved in trichothecene mycotoxin biosynthesis.

2024-01-24 | GSE233409 | GEO

Gene expression during early infection of the resistant spring wheat cultivar Wuhan1 with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the resistant spring wheat cultivar Wuhan1 inoculated with water (H2O) or Fg.

2015-06-03 | GSE54553 | GEO

Gene expression during early infection of the resistant winter wheat cultivar Dream with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the resistant winter wheat cultivar Dream inoculated with water (H2O) or Fg.

2015-06-03 | GSE54551 | GEO

Gene expression during early infection of the susceptible spring wheat cultivar Roblin with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the very susceptible spring wheat cultivar Roblin inoculated with water (H2O) or Fg.

2015-06-03 | GSE36283 | GEO

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