P53 In Inflamatory Stress Response

ABSTRACT: Activation of the p53 network plays a central role in the inflammatory stress response associated with ulcerative colitis, and may modulate cancer risk in patients afflicted with this chronic disease. The overall goal of these experiments is to study the gene expression profiles associated with four microenvironmental components of the inflammatory response (NO*, DNA damage, DNA replication arrest, and hypoxia) that result in p53 stabilization and activation. To this end, isogenic HCT116 and HCT116 TP53-/- colon cancer cells were exposed to the NO*-donor Sper/NO, H2O2 (DNA damage), hypoxia, or hydroxyurea (HU, DNA replication arrest), and their mRNA was analyzed using oligonucleotide microarrays. Cy3-labeled reference probes (untreated) and Cy5-labeled probes (samples exposed for the indic

ORGANISM(S): Homo sapiens

SUBMITTER: Ana Robles 

PROVIDER: E-GEOD-3176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress