MicroRNA Expression Profiling of Carcinoma In Situ (CIS) Cells of the Testis.

ABSTRACT: Testicular germ cell tumours (TGCTs) of young adult men, seminoma (SEM) and nonseminoma (NSEM), develop from a precursor cell, carcinoma in situ (CIS), which resembles foetal gonocytes and retains embryonic pluripotency. We used microarrays to analyse microRNA (miRNA) expression in 12 human testis samples with CIS cells and compared it to the miRNA expression profiles of normal adult testis, testis with Sertoli-cell-only (SCO) that lack germ cells, testis tumours (SEM and embryonal carcinoma, EC; an undifferentiated component of NSEM) and foetal male and female gonads. Principal component analysis revealed distinct miRNA expression profiles characteristic for each of the different tissue types. We identified several miRNAs that were unique to testis with CIS cells, foetal gonads, and testi

ORGANISM(S): Homo sapiens

SUBMITTER: Henrik Leffers 

PROVIDER: E-GEOD-31824 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress