Resolution of ntla-dependent transcriptome at 16 hpf using caged molecules

ABSTRACT: Transcription factors play diverse roles during embryonic development, combinatorially controlling multiple cellular states in a spatially and temporally defined manner. Resolving the dynamic transcriptional profiles that underlie these patterning processes is essential for understanding embryogenesis at the molecular level. Here we show how temporal, tissue-specific changes in embryonic transcription factor function can be discerned by integrating caged morpholinos (cMOs) with photoactivatable fluorophores, fluorescence-activated cell sorting (FACS), and microarray technologies. As a proof of principle, we have dynamically profiled No tail-a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, discovering and characterizing discrete sets of transcripts

ORGANISM(S): Danio rerio

SUBMITTER: James Chen 

PROVIDER: E-GEOD-31881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress