ChIP-chip from Drosophila egg chambers using MCM2-7 antibody
Ontology highlight
ABSTRACT: Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation Comparison of MCM2-7 ChIP sample compared to input DNA for Drosophila egg chambers
Project description:This SuperSeries is composed of the following subset Series: GSE31889: ChIP-chip from Drosophila egg chambers using MCM2-7 antibody GSE31890: ChIP-chip from Drosophila egg chambers using ORC2 antibody Refer to individual Series
Project description:This SuperSeries is composed of the following subset Series: GSE29517: ChIP-chip from Drosophila egg chambers using ORC2 antibody GSE29518: ChIP-chip from dissected Drosophila egg chambers using antibody recognizing RNAPII GSE29520: ChIP-chip from Drosophila egg chambers using antibody recognizing tetra-acetylated histone H4 GSE29526: Expression profile of 16C ovarian follicle cells Refer to individual Series
Project description:Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC localizes to the six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified loci Comparison of ORC2 ChIP sample compared to input DNA for two different Drosophila strains and two developmental stage samples
Project description:Chromatin immunoprecipitation of tetra-acetylated histone H4 followed by hybridization to genome-wide tiling microarrays demonstrated that tetra-acetylated H4 is enriched at the two chorion Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified loci Comparison of tetra-acetylated H4 ChIP sample compared to input DNA for two different Drosophila strains
Project description:Chromatin immunoprecipitation of RNAPII followed by hybridization to genome-wide tiling microarrays demonstrated that RNAPII localizes to all six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified genomic loci Localization of RNAPII in stage 10 egg chambers with the nurse cells manually removed
Project description:Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC does not localize to DAFC-34B after stage 10, despite a second round of replication initiation Comparison of ORC2 ChIP sample compared to input DNA for Drosophila egg chambers
Project description:We analyzed gamaH2Av ChIP-seq from hand dissected stage 10B and 13 follicle cell nuclei. Egg chambers were dissected from wild-type (OrR) or H2Av[ΔCT] ovaries to assess binding at the Drosophila Follicle Cell Amplicons and across the genome. ChIP-seq of gammaH2Av bound to follicle cell DNA from stage 10B and 13 egg chambers, collected from wild-type (OrR) and H2Av[ΔCT] Drosophila ovaries. Sequences analyzed by Illumina sequencing. Two replicates are included for each ChIP reaction.
Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of RNAPolII ChIP from third instar salivary glands relative to control IgG pulldown for two different Drosophila strains
Project description:ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of ORC2 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains
Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands. Comparison of H3K27me3 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains