Glyco-gene expression in naive and activated murine regulatory T-cells vs. conventional T-cells

ABSTRACT: In collaboration with Professor Anne Dell at the South Kensington Campus of Imperial College London, we have conducted preliminary glycomic surveys of C57BL/6 CD25+ and CD25- CD4+ T cells. Cells selected in serum-containing culture medium demonstrated unusual N-glycan profiles compared to those prepared in phosphate buffered saline (PBS). These profiles were subsequently shown to result from cellular sequestration of glycoproteins present in fetal calf serum (FCS) used to supplement the medium (1). Analysis of the N-glycan profile of murine CD4+CD25+ Tregs prepared in PBS revealed distinct differences from CD4+CD25- T cells – namely, a reduction in sialic acid capping and loss of core fucose. Preliminary studies are underway to ascertain the minimum medium required to sustain general T c

ORGANISM(S): Mus musculus

SUBMITTER: Steven Head 

PROVIDER: E-GEOD-31921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress