DNA methylation profiling of embryonic stem cell differentiation into the three germ layers [Expression analysis]

ABSTRACT: Embryogenesis is tightly regulated by multiple levels of epigenetic systems such as DNA methylation, histone modification, and chromatin remodeling. DNA methylation patterns are erased in primordial germ cells and in the interval immediately following fertilization. Subsequent reprogramming occurs by de novo methylation and demethylation. Variance of DNA methylation patterns between different cell types is not well understood. Here, using methylated DNA immunoprecipitation and tiling array technology, we have comprehensively analysed DNA methylation patterns at proximal promoter regions in mouse embryonic stem (ES) cells, ES cell-derived early germ layers (ectoderm, endoderm and mesoderm) and four adult tissues (brain, liver, skeletal muscle and sperm). Most of the methylated regions

ORGANISM(S): Mus musculus

SUBMITTER: Takayuki Isagawa 

PROVIDER: E-GEOD-32081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress