Resolving phenylalanine metabolism sheds light on natural synthesis of benzylpenicillin in Penicillium chrysogenum

ABSTRACT: Industrial production of penicillin G by Penicillium chrysogenum requires medium supplementation with the side chain precursor phenylacetate. However, P.chrysogenum grown in presence of phenylalanine as sole nitrogen source formed detectable extracellular amounts of phenylacetate and penicillin G. To get more insights in the metabolism implicated, chemostat-cultivation in presence of 13C9-phenylalanine were carried out. Quantification and modeling of the labeled metabolite pools indicated that phenylalanine was i) incorporated in nascent protein, ii) transaminated to phenylpyruvate and further converted by oxidation or by decarboxylation and iii) oxidized into tyrosine and subsequently assimilated in the homogentisate pathway. Comparative transcriptome analysis of phenylalanine and (NH4)2

ORGANISM(S): Penicillium chrysogenum

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-32097 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress