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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of CovR polymorphisms on GAS gene expression

ABSTRACT: In this study we sought to determine the effects of single amino acid replacements in the control of virulence regulatory (CovR) regulatory protein on group A streptococcal global gene expression. We compared the transcriptomes of the serotype M3 strain MGAS10870 with four derivative strains: 1) a strain in which CovR had been completely inactivated (CovR knockout); 2) a strain in which the Arg 144 amino acid was changed to a cysteine (CovR-R144C); 3) a strain in which the Arg 158 amino acid was changed to a cysteine (CovR-R158C); and 4) a strain in which the the Asn 193 amino acid was changed to an isoleucine (CovR-N193I). Duplicate samples of each strain were grown to mid-exponential phase and the relative transcriptomes were compared using an Affymetrix GeneChipl.

ORGANISM(S): Streptococcus pyogenes

SUBMITTER: Samuel Shelburne

PROVIDER: E-GEOD-32106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CovS Inactivation Reduces CovR Promoter Binding at Diverse Virulence Factor Encoding Genes in Group A Streptococcus

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