Expression data from Aspergillus niger comparing aerial structures with vegetative mycelium

ABSTRACT: A. niger undergoes dramatic changes during asexual development. We tried to identify the differences in RNA expression levels that are important for this development. We used micro-arrays to determine which genes were up- or down-regulated in the aerial structures, the part of the colony that is formed during asexual development. A. niger was grown as a sandwiched culture (Wösten et al., 1991,Journal of General Microbiology 137: 2017–2023) in a 0.25 mm layer of 0.6 % agarose between two porous polycarbonate membranes (diameter 76 mm, pore size 0.1 µm; Profiltra; www.profiltra.nl). After six days of growth, the top membrane of the sandwich was replaced by a membrane with pores of 10 µm (Profiltra), allowing formation of aerial hyphae and conidiophores for 24 h. Vegetative mycelium and aeria

ORGANISM(S): Aspergillus niger

SUBMITTER: R Bleichrodt 

PROVIDER: E-GEOD-32123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress