Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Peripheral blood mononuclear cells, control vs. Kashin-Beck Disease

ABSTRACT: Objective: To investigate the differences between the gene expression profiles in peripheral blood mononuclear cells (PBMC) from normal controls and patients with Kashin-Beck disease (KBD). Methods: Twenty KBD patients and 12 normal subjects were selected from a KBD-endemic area and divided into four pairs of KBD vs. control (KBD, n=5 per pair; control, n=3 per pair). RNAs were respectively isolated from KBD PBMCs and normal PBMCs. Gene expression profiles were analyzed by oligonucleotide microarray. Two-condition experiment, control vs. KBD PBM cells. Biological replicates: 4 control replicates, 4 KBD replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: xiaoming wu

PROVIDER: E-GEOD-32127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Peripheral blood mononuclear cells, control vs. Kashin-Beck Disease

Project description:Objective: To investigate the differences between the gene expression profiles in peripheral blood mononuclear cells (PBMC) from normal controls and patients with Kashin-Beck disease (KBD). Methods: Twenty KBD patients and 12 normal subjects were selected from a KBD-endemic area and divided into four pairs of KBD vs. control (KBD, n=5 per pair; control, n=3 per pair). RNAs were respectively isolated from KBD PBMCs and normal PBMCs. Gene expression profiles were analyzed by oligonucleotide microarray.

2011-09-23 | GSE32127 | GEO

Human Peripheral blood mononuclear cells (PBMCs): Control vs. Kashin-Beck Disease

Project description:Objective:To identify an accurate blood-based gene signature for early detection of Kashin-Beck disease (KBD). Methods: Gene expression analysis was conducted of peripheral blood samples from 100 patients with KBD and 100 controls randomly chosen from two KBD-endemic areas Two-condition experiment, Control vs. KBD PBM cells. Biological replicates: 100 control replicates, 100 KBD replicates.

2014-07-16 | E-GEOD-59446 | biostudies-arrayexpress

Human Peripheral blood mononuclear cells (PBMCs): Control vs. Kashin-Beck Disease

2014-07-16 | GSE59446 | GEO

Genome-wide Gene Expression profiles of Kashin-Beck Disease

Project description:We compared genome-wide gene expression profiles of articular cartilage derived from 4 KBD patients and 4 normal controls. Total RNA was isolated from cartilage samples following by being amplified, labeled and hybridized to Agilent human 1A 22k microarray chip(G4110B).

2011-10-01 | E-MEXP-3196 | biostudies-arrayexpress

circulating microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis

Project description:We compared the circulating microRNA expression profiles of KBD, osteoarthritis (OA), rheumatoid arthritis (RA) and healthy controls. Blood specimens were collected from 3 KBD patients, 3 OA patients, 3 RA patients and 3 healthy controls. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array.

2016-06-01 | E-MTAB-3514 | biostudies-arrayexpress

Genome-Wide Differentially Methylated Region Analysis to Reveal Epigenetic Differences of Articular Cartilage in Kashin-Beck Disease and Osteoarthritis

Project description:Kashin-Beck disease (KBD) is a degenerative osteoarticular disorder, and displays the significant differences with osteoarthritis (OA) regarding the etiology and molecular changes in articular cartilage. However, the underlying dysfunctions of molecular mechanisms in KBD and OA remain unclear. Here, we performed the various genome-wide differentially methylated region analyses to reveal the distinct differentially methylated regions (DMRs) and corresponding differentially methylated genes (DMGs), and enriched functional pathways/GO terms for KBD and OA.

2020-12-03 | GSE162484 | GEO

Integration of miRNA in exosomes and single-cell RNA-seq profiles in endemic osteoarthritis, Kashin-Beck disease

Project description:Kashin-Beck disease (KBD) is an endemic, chronic degenerative joint disease in China. Exosomes miRNAs, as signaling molecules in intercellular communication, can transfer specific biological martials into target cell to regulate the their function and might participate in the pathogenesis of KBD. We isolated serum and chondrocytes-derived exosomes, miRNA sequencing revealed exosomes miRNA profiles and differentially expressed miRNAs (DE-miRNAs) were identified. The target genes were predicted of known and novel DE-miRNAs with TargetScan 5.0 and miRanda 3.3a database. Single-cell RNA sequencing (scRNA-seq) was performed to identify chondrocyte clusters and their gene signatures in KBD. And we performed comparative analysis between the serum and chondrocytes-derived exosomes DE-miRNA target genes and differentially expressed genes of each cell clusters.A total of 20 DE-miRNAs were identified in serum-derived exosomes. In the miRNA expression of chondrocytes-derived exosomes, 53 DE-miRNAs were identified. 16063 predicted targets were identified as the target genes in the serum-derived exosomes, 57316 predicted targets were identified as the target genes in the chondrocytes-derived exosomes. Seven clusters were labelled by cell type according to the expression of previously described markers. 315 common genes were found among serum/chondrocytes-derived exosomes DE-miRNA target genes and DEGs identified by scRNA-seq analysis. We firstly integratly analyzed the serum and chondrocytes exosomes miRNA with single-cell RNA sequencing (scRNA-seq) data of KBD chondrocyte, the results showed that DE-miRNAs in exosomes might play a potential role in regulating genes expression in different KBD chondrocytes clusters by exosomes mediating cell-cell communications functions, which could improve the new diagnosis and treatment methods for KBD.

2023-11-01 | GSE246097 | GEO

Comprehensive expression profiles of mRNAs, lncRNAs and miRNAs in Kashin-Beck Disease identified by RNA-sequencing

Project description:RNA‐seq technology to detect the differentially expressed mRNAs, lncRNAs and miRNAs in KBD patients. The database of Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEEG) were employed to conduct functional annotation and pathway enriched analysis for differential expression.

2021-10-27 | GSE186593 | GEO

Serum proteomics of patients with Kashin-beck disease

Project description:Kashin-Beck disease (KBD), a chronic and endemic degenerative chronic joint disease, which etiolo. In order to discover biomarkers for KBD, the serum DIA proteomics analysis was conducted to detect the differentially expressed proteins between KBD patients and healthy controls.

2023-03-02 | PXD040595 | iProX

Prevalence of hand osteoarthritis and knee osteoarthritis in Kashin-Beck disease endemic areas and non Kashin-Beck disease endemic areas: A status survey.

Project description: Not available

| S-EPMC5761882 | biostudies-literature