Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Ischemic vs. nephrotoxic acute renal failure, early time points (2h and 8h)

ABSTRACT: Acute renal failure (ARF) has high morbidity and mortality. In animal ARF models, effective treatments must be administered before or shortly after the insult, limiting their clinical potential. We used microarrays to identify early biomarkers that distinguish ischemic from nephrotoxic ARF, or that detect both injury types. We compared rat kidney transcriptomes 2 and 8 hours after ischemia/reperfusion and after mercuric chloride. Quality control and statistical analyses were necessary to normalize inter-experimental groups, eliminate outliers, and exclude unaltered genes. Principal component analysis revealed distinct ischemic and nephrotoxic trajectories, and clear array groupings. Therefore, we used supervised analysis, t-tests and fold changes, to compile gene lists for each group, exclusive or non-exclusive, alone or in combination. We used two lots of microarrays (Lot 1, n = 24, Lot 2, n = 12), for a total of 36 microarrays. Five of them were duplicates, where two aliquots of RNA from the same rat were processed independently and hybridized to separate microarrays. Normal rats were used in both lots (n = 3 for each lot) for normalization.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Peter Yuen

PROVIDER: E-GEOD-3219 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ischemic and nephrotoxic acute renal failure are distinguished by their broad transcriptomic responses.

Yuen Peter S T PS Jo Sang-Kyung SK Holly Mikaela K MK Hu Xuzhen X Star Robert A RA

Physiological genomics 20060228 3

Acute renal failure (ARF) has a high morbidity and mortality. In animal ARF models, effective treatments must be administered before or shortly after the insult, limiting their clinical potential. We used microarrays to identify early biomarkers that distinguish ischemic from nephrotoxic ARF or biomarkers that detect both injury types. We compared rat kidney transcriptomes at 2 and 8 h after ischemia/reperfusion and after mercuric chloride. Quality control and statistical analyses were necessary ...[more]

PMID: 16507785

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Dataset Information

Ischemic vs. nephrotoxic acute renal failure, early time points (2h and 8h)

Publications

Ischemic and nephrotoxic acute renal failure are distinguished by their broad transcriptomic responses.

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