Transcriptome analysis of the Tap efflux pump mutant in Mycobacterium bovis BCG.

ABSTRACT: We used DNA microarrays to define the physiological roles of the Tap efflux pump in M. bovis BCG during the exponential and the stationary phase of in vitro growth. For this purpose we constructed a M. bovis BCG strain in which the tap gene was inactivated by the insertion of a hygromycin resistance cassette (?-hyg). When the gene expression patterns of the tap mutant were compared to the wild-type strain, almost no differences were observed during exponential growth; only seven genes slightly increased their expression. In contrast, more that 100 genes showed a variation in their level of expression during stationary growth. More than ten representative genes were chosen from the microarray experiments and their expression was measured by quantitative RT-PCR using sigA as invariant intern

ORGANISM(S): Mycobacterium bovis BCG

SUBMITTER: Santiago Ramon-Garcia 

PROVIDER: E-GEOD-32249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress