High-throughput RT-PCR analysis of Colon Adenocarcinoma Stem Cell Lines
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ABSTRACT: Human early stage colon adenocarcinoma stem cell line was FACS-sorted for CCR9- and CCR9+ cell groups and differential expression was identified via Wafergen SmartChip RT-PCR gene expression profiling. CCR9- and CCR+ cells were FACS-sorted. 24 hours afterwards, cells were treated with 100ng/ml human CCL25 for 30 min. RNA was extracted from both populations using PureLink RNA Mini kit (Invitrogen) and analyzed using the SmartChip Real-Time PCR System
ORGANISM(S): Homo sapiens
SUBMITTER: Zeynep Gumus
PROVIDER: E-GEOD-32290 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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