High-throughput sequencing of small RNAs in Chlamydomonas reinhardtii

ABSTRACT: Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from cultures of Chlamydomonas reinhardtii (in control, phosphate starvation and sulphate starvation conditions). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Emanuele De Paoli 

PROVIDER: E-GEOD-32457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress