Combinatorial patterning of chromatin regulators uncovered by genome-wide location analysis in human cells

ABSTRACT: Hundreds of Chromatin Regulators (CRs) control chromatin structure and function by catalyzing and binding histone modifications, yet the rules governing these key processes remain obscure. Here, we present a systematic approach to infer CR function. We developed ChIP-string, a meso-scale assay that combines chromatin immunoprecipitation with a signature readout of 487 representative loci. We applied ChIP-string to screen 145 antibodies, thereby identifying effective reagents, which we used to map the genome-wide binding of 29 CRs in two cell types. We found that specific combinations of CRs co-localize in characteristic patterns at distinct chromatin environments, genes of coherent functions and distal regulatory elements. When comparing between cell types, CRs redistribute to different lo

ORGANISM(S): Homo sapiens

SUBMITTER: Bradley Bernstein 

PROVIDER: E-GEOD-32509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress