To investigate how the glycosylation of podocyte proteins changes during diabetic kidney disease

ABSTRACT: Podocytes are cells of the visceral epithelium in the kidneys and form a crucial component of the glomerular filtration barrier, contributing to size selectivity and maintaining a massive filtration surface. We are interested in pursuing a microarray analysis to identify the glycosylation-related genes that are modulated in podocytes during insulin and glucose stimulation. As such, we propose to isolate RNA from cultured AB 8/13 human podocyte cell line in their normal state and treated with different concentrations of insulin and glucose. Initially, we would like to analyze 12 samples (Normal control, 2 Starved controls, Insulin experiments 1-4, and glucose experiments 1-5). These experiments would give us information on the gene regulation changes in different healthy and diabetic condit

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Head 

PROVIDER: E-GEOD-32553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress