Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Molecular crosstalk between hepatocytes and hepatic stellate cells.

ABSTRACT: Aim of the study was to characterize at a molecular level (changes in transcriptomes) the crosstalk between tumor hepatocytes and activated hepatic stellate cells (HSC) in liver cancer. This was adressed by using a coculture model system of HepaRG cell line (tumor hepatocytes, human), and LX2 cell line (HSC, human). By using genome-wide expression profiling, we demonstrated that hepatocyte-HSC crosstalk is bidirectional and results in the deregulation of functionally relevant gene networks. HepaRG and LX2 cells were cultured alone in serum- and DMSO-free William's E medium or together using 1 M-BM-5m pore size transwell inserts which allow diffusion of media components but prevent cell migration (BD Biosciences, San Jose, CA). Triplicate experiments were performed: HepaRG (culture versus coculture), LX2 (culture versus coculture).

ORGANISM(S): Homo sapiens

SUBMITTER: Cedric Coulouarn 

PROVIDER: E-GEOD-32565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Hepatocyte-stellate cell cross-talk in the liver engenders a permissive inflammatory microenvironment that drives progression in hepatocellular carcinoma.

Coulouarn Cédric C   Corlu Anne A   Glaise Denise D   Guénon Isabelle I   Thorgeirsson Snorri S SS   Clément Bruno B  

Cancer research 20120314 10

Many solid malignant tumors arise on a background of inflamed and/or fibrotic tissues, features that are found in more than 80% hepatocellular carcinomas (HCC). Activated hepatic stellate cells (HSC) play a critical role in fibrogenesis associated with HCC onset and progression, yet their functional impact on hepatocyte fate remains largely unexplored. Here, we used a coculture model to investigate the cross-talk between hepatocytes (human hepatoma cells) and activated human HSCs. Unsupervised g  ...[more]

Similar Datasets

Transcriptomics Molecular crosstalk between hepatocytes and hepatic stellate cells.
2012-06-01 | GSE32565 | GEO
Transcriptomics Communication between the HCCLM3 cells and LX2 cells in a 3D tumor-on-a-chip
2023-07-31 | GSE208007 | GEO
Transcriptomics Early target genes of aflatoxin B1 in human hepatic cells
2010-12-07 | E-GEOD-25844 | biostudies-arrayexpress
Transcriptomics Comparative gene expression profiles induced by PPARM-NM-3 and PPARM-NM-1/M-NM-3 agonists in human hepatocytes
2014-02-09 | E-GEOD-27183 | biostudies-arrayexpress
Transcriptomics Gut-Liver Microphysiological Systems Revealed Arachidonic Acid as a Potential Crosstalk Factor Modulating Drug Metabolism
2023-11-01 | GSE244407 | GEO
Genomics,Multiomics Homo sapiens
| PRJNA230634 | ENA
Proteomics Proteome and secretome of HepaRG cells
2019-02-27 | PXD010304 | Pride
Transcriptomics Inter-individual variability in gene expression profiles in human hepatocytes and comparison with HepaRG cells
2015-10-01 | GSE41289 | GEO
Transcriptomics Effect of Microcystin-LR and cylindrospermopsin (CYN) on gene expression in human hepatocytes, HepaRG cells and HCC HepG2 cells.
2023-05-12 | E-MTAB-12881 | biostudies-arrayexpress
Transcriptomics Effect of depletion of YAP and TAZ on gene expression of LX2 cells
2023-11-30 | GSE219088 | GEO