Transcription profiling of rat olfactory ensheathing cells (OEC) comparison of three populations

ABSTRACT: Comparison of three populations of OECs derived from the ONL of rats. The three populations were cultured employing the same culture medium but were maintained in vitro for a different amount of time (number of population doublings or PDs). OEC Ep cells were maintained for less than one week in vitro (less than 3 PDs); OEC Lp were maintained for over a year (more than 50 PDs) and TEG3 OEC cell line has been serially passed in culture for long-periods of time after genetic immortalization. Experiment Overall Design: RNA sample preparation and experimental design. Total RNA was isolated with TRIZOL (Invitrogen, Gibco-BRL) from primary OEC cultures (OEC Ep and Lp) and the OEC cell line (TEG3). Cultured cells were harvested at confluency with a rubber scrapper, resuspended in the TRIZOL reagen

ORGANISM(S): Rattus norvegicus

SUBMITTER: Erika Pastrana 

PROVIDER: E-GEOD-3258 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress