A genome wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation

ABSTRACT: Despite intense investigation of intrinsic and extrinsic factors that regulate pluripotency, the process of initial fate commitment of embryonic stem (ES) cells is still poorly understood. Here, we used a genome wide shRNA screen in mouse ES cells to identify genes that are essential for initiation of differentiation. Knockdown of the scaffolding protein Mek binding protein 1 (Mp1, also known as Lamtor3, Map2k1ip1) stimulated self-renewal of ES cells, blocked differentiation and promoted proliferation. Fibroblast growth factor 4 (FGF4) signaling is required for initial fate commitment of ES cells. Knockdown of Mp1 inhibited FGF4-induced differentiation but did not alter FGF4 driven proliferation. This uncoupling of differentiation and proliferation was also observed when oncogenic Ras isof

ORGANISM(S): Mus musculus

SUBMITTER: Bart A. Westerman 

PROVIDER: E-GEOD-32595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress