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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Global gene expression analysis in murine iPS cells derived with mouse, chick and zebrafish Nanog orthologs

ABSTRACT: Nanog null neural stem (NS) cells were reprogrammed to naive pluripotency in 2i/LIF conditions with chick (c) and zebrafish (z) Nanog orthologs. Global gene expression was compared to iPS cells derived with mouse (m) Nanog. Murine iPS cells derived with zebrafish nanog, chick nanog, and mouse nanog orthologs (2 replicates each).

ORGANISM(S): Mus musculus

SUBMITTER: Thorold Theunissen

PROVIDER: E-GEOD-32650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression analysis in murine iPS cells derived with Nanog orthologs

Project description:This SuperSeries is composed of the following subset Series: GSE32464: Global gene expression analysis in murine iPS cells derived with mouse and human Nanog orthologs GSE32650: Global gene expression analysis in murine iPS cells derived with mouse, chick and zebrafish Nanog orthologs Refer to individual Series

2011-10-23 | E-GEOD-32715 | biostudies-arrayexpress

Global gene expression analysis in murine iPS cells derived with mouse and human Nanog orthologs

Project description:Nanog null neural stem (NS) cells were reprogrammed to naive pluripotency in 2i/LIF conditions with mouse (m) Nanog and human (h) Nanog. Global gene expression in resulting iPS cells was compared to embryonic stem (ES) cells and nanog null NS cells. Murine iPS cells derived with mouse nanog iPS and human nanog iPS and then compared to embryonic stem cells and nanog null neural stem cells (3 replicates each).

2011-10-23 | E-GEOD-32464 | biostudies-arrayexpress

Global gene expression analysis in murine iPS cells derived with mouse, chick and zebrafish Nanog orthologs

Project description:Nanog null neural stem (NS) cells were reprogrammed to naive pluripotency in 2i/LIF conditions with chick (c) and zebrafish (z) Nanog orthologs. Global gene expression was compared to iPS cells derived with mouse (m) Nanog.

2011-10-24 | GSE32650 | GEO

Mus musculus

Project description:Global gene expression analysis in murine iPS cells derived with mouse, chick and zebrafish Nanog orthologs

| PRJNA154473 | ENA

Global gene expression analysis in murine iPS cells derived with mouse and human Nanog orthologs

2011-10-24 | GSE32464 | GEO

Project description:Global gene expression analysis in murine iPS cells derived with Nanog orthologs

| PRJNA146981 | ENA

Global gene expression analysis of Ncoa3 knockdown in mouse embryonic stem cells

Project description:Orphan nuclear receptor Esrrb is vital in maintaining ES cells and like Oct4, Sox2 and Nanog is essential for self-renewal and pluripotency. Esrrb functions in somatic cells via LBD/AF-2-dependent coactivator recruitment to target genes. Here we show that in ES cells coactivator recruitment is similarly required and identify Ncoa3 as the Esrrb coactivator needed for activation of its target genes. Ncoa3 is essential for self-renewal and the induction of pluripotency in reprogramming, and genome-wide analysis of Ncoa3 binding reveals extensive overlap with Esrrb and pluripotency factors along with marks of active genes. Mechanistically, we show Ncoa3 is specifically required to bridge RNApol2 to Esrrb. We thus identify a new member of the ES pluripotency network and describe Esrrb and Ncoa3 as key factors linking core pluripotency factors to the general transcription machinery. Three biological replicates each for control scrambled shRNA and Ncoa3 shRNA transfected E14 mouse ESCs. The global gene expression profiles of Ncoa3 knockdown cells were compared to control scrambled shRNA knockdown cells 4 days post-transfection.

2012-10-02 | E-GEOD-40192 | biostudies-arrayexpress

Mus musculus

Project description:Global gene expression analysis in murine iPS cells derived with mouse and human Nanog orthologs

| PRJNA154537 | ENA

Project description:zebrafish nanog mutant sequencing

| PRJNA633216 | ENA

Generation of human induced pluripotent stem cells from mesenchymal cells of gut mesentery by Oct4/Sox2/Nanog

Project description:Background and aim: Human Induced pluripotent stem (iPS) cells have been derived from dermal ﬁbroblasts, keratinocytes and blood cells by ectopic expression of defined transcription factors.1–5 Application of this approach in human cells would have enormous potential and generate patient-specific pluripotent stem cells to accelerate the implementation of stem cells for clinical treatment of degenerative diseases. In the present study, we investigated whether genetically marked human mesenchymal cells of gut mesentery may give rise to iPS cells. Methods: We used lentiviruses to express Oct4, Sox2, Nanog in mesenchymal cells of gut mesentery, then generated iPS cells were identified in many aspects including morphology, pluripotent markers, global gene expression profile, DNA methylation status at pluripotent cell-speciﬁc genes, embryoid bodies and terotomas formation. Results: The resulting iPS cells from mesenchymal cells of gut mesentery were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, and epigenetic status of pluripotent cell-speciﬁc genes. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. DNA ﬁngerprinting showed that the human iPS cells were derived from the donor cells and are not a result of contamination. one sample/variable

2009-09-26 | E-GEOD-18180 | biostudies-arrayexpress

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