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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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DIFFERENTIAL GENE EXPRESSION OF MEDULLARY THYROID CARCINOMA REVEALS SPECIFIC MARKERS ASSOCIATED WITH GENETIC CONDITIONS.

ABSTRACT: Transcriptional analysis of 49 primary medullary thyroid carcinoma tumors. Comparisons MTCM918T vs MTC634 and MTCM918T vs MTCWT. 49 (52 hybridized tumors with 3 replicates) primary Medullary Thyroid Carcinoma (MTC) cases were hybridized onto a cDNA microarray in order to identify the unique markers for specific genetic classes of MTC.

ORGANISM(S): Homo sapiens

SUBMITTER: Mercedes Robledo

PROVIDER: E-GEOD-32662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential gene expression of medullary thyroid carcinoma reveals specific markers associated with genetic conditions.

Maliszewska Agnieszka A Leandro-Garcia Luis J LJ Castelblanco Esmeralda E Macià Anna A de Cubas Aguirre A Goméz-López Gonzalo G Inglada-Pérez Lucía L Inglada-Pérez Lucía L Álvarez-Escolá Cristina C De la Vega Leticia L Letón Rocío R Gómez-Graña Álvaro Á Landa Iñigo I Cascón Alberto A Rodríguez-Antona Cristina C Borrego Salud S Zane Mariangela M Schiavi Francesca F Merante-Boschin Isabella I Pelizzo Maria R MR Pisano David G DG Opocher Giuseppe G Matias-Guiu Xavier X Encinas Mario M Robledo Mercedes M

The American journal of pathology 20121128 2

Medullary thyroid carcinoma accounts for 2% to 5% of thyroid malignancies, of which 75% are sporadic and the remaining 25% are hereditary and related to multiple endocrine neoplasia type 2 syndrome. Despite a genotype-phenotype correlation with specific germline RET mutations, knowledge of pathways specifically associated with each mutation and with non-RET-mutated sporadic MTC remains lacking. Gene expression patterns have provided a tool for identifying molecular events related to specific tum ...[more]

PMID: 23201134

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Project description:Transcriptional analysis of 49 primary medullary thyroid carcinoma tumors. Comparisons MTCM918T vs MTC634 and MTCM918T vs MTCWT.

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Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma [methylation component]

Project description:Medullary thyroid carcinoma (MTC) accounts for 1-2% of thyroid malignancies. It is a disease of few genetic drivers, and the etiology specifically associated with each mutation remains unknown. Here, we investigated the role of aberrant DNA methylation in the MTC development. We performed genome-wide DNA methylation profiling assessing >27,000 CpGs in the largest MTC series reported to date, comprising 48 molecularly characterized tumors. We observed significant differences between the methylation patterns among the samples bearing the RETM918T, RETC634 mutation and “wild-type” (WT) tumors, but not those RAS-related. In detail, the RETM918T –related tumors had a larger number of hypomethylation events when compared to RETC634 – positive and WT ones. Moreover, through the integration of methylation with mRNA and miRNA expression data of the same tumors, we identified genes whose expression is negatively correlated with the methylation status of their promoters. For PLCB2, DKK4, MMP20 as well as miR-10a, -30a and -200c, the impact of promoter methylation levels on expression of these genes in MZ-CRC-1 and TT cell lines was assessed. Finally, the aberrant methylation validation of three of the genes in an independent set of 25 MTCs by bisulfite pyrosequencing suggests their role as MTC methylation markers.

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Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma [miRNA component]

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Transcriptional Targeting Of Oncogene Addiction In Medullary Thyroid Cancer [RNA-Seq]

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