Time-course effect of estradiol and estradiol-BSA on early gene expression in MDA-MB-231 cells

ABSTRACT: Estrogens have been reported to activate several processes via membrane binding to either classic estrogen receptors (ERs) or GPR30. We have used either estradiol or BSA-conjugated estradiol in order to initiate membrane-initiated actions and ICI 172,780 (ICI) or G15 to explore ER- and GPR30-related transcription. Our results show that the majority of G15-inhibited transcription is depending on ERs, as it is also inhibited by ICI. However, a small number of transcripts, related to specific actions/pathways is either exclusively inhibited by G15, providing evidence about a specific GPR30 signature, or not inhibited by ICI or G15 suggesting the existence of another, yet unidentified estrogen receptor. Cells after a 4h incubation with medium containing 10% charcoal stripped FBS were incubated

ORGANISM(S): Homo sapiens

SUBMITTER: George Notas 

PROVIDER: E-GEOD-32668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress