Analysis of copy number changes primary and metastatic colorectal cancer samples

ABSTRACT: Structural rearrangements form a major class of somatic variation in cancer genomes. Local chromosome shattering, termed chromothripsis, is a mechanism proposed to be the cause of clustered chromosomal rearrangements and was recently described to occur in a small percentage of tumors. The significance of these clusters for tumor development or metastatic spread is largely unclear. We used genome-wide long mate-pair sequencing and SNP array profiling to reveal that chromothripsis is a widespread phenomenon in primary colorectal cancer and metastases. We find large and small chromothripsis events in nearly every colorectal tumor sample and show that several breakpoints of chromothripsis clusters and isolated rearrangements affect cancer genes, including NOTCH2, EXO1 and MLL3. We complemented

ORGANISM(S): Homo sapiens

SUBMITTER: Wigard Kloosterman 

PROVIDER: E-GEOD-32711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress