Characteristic genome rearrangements in experimental evolution

ABSTRACT: Genome rearrangements, especially amplifications and deletions, have regularly been observed as responses to sustained application of the same strong selective pressure in microbial populations growing in continuous culture. We studied eight strains of budding yeast (Saccharomyces cerevisiae) isolated after 100–500 generations of growth in glucose-limited chemostats. Changes in DNA copy number were assessed at single-gene resolution by using DNA microarray-based comparative genomic hybridization. Six of these evolved strains were aneuploid as the result of gross chromosomal rearrangements. Most of the aneuploid regions were the result of translocations, including three instances of a shared breakpoint on chromosome 14 immediately adjacent to CIT1, which encodes the citrate synthase that pe

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Maitreya Dunham 

PROVIDER: E-GEOD-3285 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress