Conserved Function of lincRNAs in Vertebrate Embryonic Development Despite Rapid Sequence Evolution

ABSTRACT: Thousands of large intervening non-coding RNAs (lincRNAs) have been identified in mammals. To better understand the evolution and functions of these enigmatic RNAs, we used chromatin marks, poly(A)-site mapping and RNA-Seq data, to identify more than 550 distinct lincRNAs in zebrafish. Although these shared many characteristics with mammalian lincRNAs, only 29 had detectable sequence similarity with putative mammalian orthologs, typically restricted to a single short region of high conservation. Other lincRNAs had conserved genomic locations without detectable sequence conservation. Antisense reagents targeting conserved regions of two zebrafish lincRNAs caused developmental defects. Reagents targeting splice sites caused the same defects and were rescued by adding either the mature lincRN

ORGANISM(S): Danio rerio

SUBMITTER: Igor Ulitsky 

PROVIDER: E-GEOD-32880 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress