Identification of Yeast Genes that Confer Resistance to Chitosan Oligosaccharide (COS) Using Chemogenomics

ABSTRACT: Abstract: Chemogenomic fitness assays were combined with a transcriptome analysis to understand both the mode of action and the mechanisms of resistance to Chitosan oligosaccharides (COS). COS are deacetylated chitin compounds, with antimicrobial properties, that are presumed to act by disrupting the cell membrane. The fitness assays identified 39 yeast deletion strains sensitive to COS and 21 suppressors of COS sensitivity. The genes identified encode membrane proteins and members of the protein degradation/ proteosome pathway. The transcriptomes of wild-type and five suppressor strains overexpressing ARL1, BCK2, ERG24, MSG5, or RBA50, were analyzed in the presence and absence of COS. The COS-induced transcriptional response is distinct from previously described environmental stress res

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Maria Jaime 

PROVIDER: E-GEOD-32888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress