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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat ductus arteriosus and aorta for comparison of pre and post birth oxygen effects

ABSTRACT: Compared the gene expression profiles of two contiguous vessels pre and post - birth, namely the Ductus Arteriosus (DA), committed to become a fibrous ligament, and the Aorta (Ao), which conversely takes on a permanent activity. The effect of artificially enhanced O2 tension was analyzed in DA in the pre-birth condition.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Flavio Coceani

PROVIDER: E-GEOD-3290 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in ductus arteriosus and aorta: comparison of birth and oxygen effects.

Costa Mario M Barogi Silvia S Socci Nicholas D ND Angeloni Debora D Maffei Margherita M Baragatti Barbara B Chiellini Chiara C Grasso Emanuela E Coceani Flavio F

Physiological genomics 20060117 2

Ductus arteriosus (DA) closure is initiated by oxygen rise postnatally and progresses in two, functional-to-permanent, stages. Here, using GeneChip Arrays in rats (normoxic and hyperoxic fetus, normoxic newborn), we examined whether oxygen alone duplicates the birth process in affecting DA genes. In addition, by comparing DA with aorta (Ao), we identified features in postnatal gene profile marking transitional adjustments in a closing (DA) vs. a persistent (Ao) vessel. We found changes in neonat ...[more]

PMID: 16418320

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Expression profiling in perinatal Ductus Arteriosus (DA) and Aorta (Ao).

Project description:Compared the gene expression profiles of two contiguous vessels pre and post - birth, namely the Ductus Arteriosus (DA), committed to become a fibrous ligament, and the Aorta (Ao), which conversely takes on a permanent activity. The effect of artificially enhanced O2 tension was analyzed in DA in the pre-birth condition. Keywords: Comparison gene expression

2005-09-13 | GSE3290 | GEO

Transcriptional profiles in the chicken ductus arteriosus during hatching

Project description:The ductus arteriosus constricts after birth or hatching and eventually closes to terminate embryonic circulation. Chicken embryos have two long ductus arteriosus. Then the pulmonary artery-sided and descending aorta-sided ductus arteriosus have distinct functional characteristics, such as oxygen responsiveness. We used microarrays to elucidate the difference between the pulmonary artery-sided and descending aorta-sided ductus arteriosus in their transcriptional profiles.

2018-09-19 | GSE120116 | GEO

Microarray gene expression analysis in ovine ductus arteriosus during fetal development and birth transition.

Project description:Patent ductus arteriosus (PDA) in the newborn is the most common congenital heart anomaly, and is significantly more common in preterm infants. Contemporary pharmacological treatment is effective in only 70 to 80% of the cases. Moreover, indomethacin or ibuprofen, which are used to close a PDA may be accompanied by serious side effects in premature infants. To explore the novel molecular pathways, which may be involved in the maturation and closure of the ductus arteriosus (DA), we used fetal and neonatal sheep to test the hypothesis that maturational development of DA is associated with significant alterations in specific mRNA expression.

2016-10-12 | GSE87840 | GEO

Gene Profiling in Mouse Fetal Ductus Arteriosus

2014-08-05 | GSE51664 | GEO

Expression profiling in ductus arteriosus and aorta of Brown-Norway and Fischer344 rats

2015-08-29 | GSE40534 | GEO

Expression profiling in ductus arteriosus and aorta of Brown-Norway and Fischer344 rats

Project description:Comparison of gene expression levels in ductus arteriosus (DA) and aorta in full-term (21 days) neonates of Brown-Norway (BN) and Fischer344 (F344) rats We analyzed the fold difference between BN and F344 rats in ductus arteriosus in order to identify the down-regulated and up-regulated genes specifically in BN rat's DA. The differences in aorta was also examined for additional comparison. Total RNA was extracted from aorta and DA tissues from BN and F344 rat neonates one hour after delivery, and converted to biotin-labeled cRNAs that were hybridized to Affymetrix Rat Gene 1.0 ST Array.

2015-08-29 | E-GEOD-40534 | biostudies-arrayexpress

Gene Profiling in Mouse Fetal Ductus Arteriosus

Project description:The ductus arteriosus (DA) is a fetal vascular shunt that is located between the main pulmonary artery and the aorta. Oxygenated fetal blood from the placenta is shunted past the uninflated fetal lungs, crosses the DA, and is then available to the peripheral organs. In utero closure of the DA is deleterious, but postnatal closure of the DA is necessary for establishment of pulmonary circulation and the transition to newborn life. We used microarrays to compare ductus arteriosis (DA) and aortic gene expression at a single time point (day 19 of gestation, which is the day of expected delivery). DA and corresponding aortic vascular segments from fetal mice on day 19 of gestation were collected from every pup in a litter (up to the first 10 pups). These samples were pooled and considered representative of the DA's or aortas from one pregnant female. Tissues were homogenized via 27g needle, per GentraSystems Versagene kit

2014-08-05 | E-GEOD-51664 | biostudies-arrayexpress

Human plasma LC-MSMS - Altered plasma proteins released from platelets and endothelial cells are associated with human patent ductus arteriosus

Project description:Patent ductus arteriosus (PDA) is the third most common congenital heart disease and resulted from the persistence of ductal patency after birth. Ductus arteriosus closure involves functional and structural remodeling, controlled by many factors. The closure-related human plasma proteins are unknown. Here we for the first time demonstrate six key differential plasma proteins in the human PDA patients using proteomic technology and present a model to illustrate the constriction and closure of ductus arteriosus. We found that permanent closure might be regulated by the proteins related to platelet activation and coagulation cascades, complement mannan-binding-lectin, and other systemic signaling pathways. Our findings indicate that the differential proteins involved in these pathways may play key roles in the non-closure of the ductus arteriosus and may be developed as biomarkers for diagnosis. All those findings may be served as the basis of understanding the etiology and pathogenesis of PDA.

2018-11-30 | PXD008568 | Pride

Expression data from endthelial cells in ductus arteriosus and aorta in rat fetuses or neonates

Project description:Endothelial cells (Ecs) lining the blood vessels have been known to have a variety of functions and play a central role in homeostasis of the circulatory system. Since ductus arteriosus (DA) has unique characteristics comparing other vessels, we hypothesized that ECs were the primary target to investigate the molecular mechanisms of DA-specific vasoconstriction and vascular remodeling. Using fluorescence activated cell sorter, we isolated ECs from pooled tissues from the DA or the aorta of Wistar rat fetuses on the 21st day of gestation (e21) or 30 minutes after birth (day0). We prepared 3 sets of ECs in each tissues and time points. We aimed to compare the gene profiles of DA and the aorta at each developmental stage.

2013-10-01 | E-GEOD-40500 | biostudies-arrayexpress

Transcriptional evaluation of the ductus arteriosus at the single cell level uncovers a requirement for vimentin for complete closure

Project description:Failure to close the ductus arteriosus immediately post-birth, patent ductus arteriosus (PDA), accounts for up to 10% of all congenital heart defects. Despite significant advances in PDA management options, including pharmacological treatment targeting the prostaglandin pathway, a proportion of patients fail to respond and must undergo surgical intervention. Thus, further refinement of the cellular and molecular mechanisms that govern vascular remodeling of this vessel is required. As anticipated, single-cell RNA sequencing on the ductus arteriosus in mouse embryos at E18.5, P0.5, and P5, revealed broad transcriptional alterations in the endothelial, smooth muscle, and fibroblast cell compartments. After close evaluation of the transcriptomic dataset, we predicted that vimentin might be required for complete closure of the vessel. Subsequent studies demonstrated that, in fact, mice with genetic deletion of vimentin fail to fully remodel the ductus arteriosus. Through single-cell RNA-sequencing and by tracking closure of the ductus arteriosus postnatally in mice, we uncovered the unexpected contribution of vimentin in driving complete closure of the ductus arteriosus potentially through regulation of the Notch signaling pathway.

2022-09-26 | GSE188202 | GEO

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