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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Oxidative damage and respiratory burst in Multiple Sclerosis

ABSTRACT: In this project we focused on white matter injury in relapsing remitting MS. We concentrated on material obtained from patients who suffered from fulminant active MS to identify possible sources for ROS production in relation to demyelination and neurodegeneration. To identify possible sources for ROS production in relation to demyelination and neurodegeneration in MS, we used genome wide microarray analysis of gene expression in carefully dissected lesion areas of patients with fulminant acute MS. Preceding the gene expression profiling all cases were characterized histologically and areas of interest were identified. We included 4 control cases, 2 active MS cases were we separated 3 distinct lesions areas (NAWM, initial lesion and early demyelinated lesion) and 1 active MS case were we separated 2 distinct lesion areas (NAWM and early demyelinated lesion). The RNA was isolated from those areas, amplified and hybridized to Agilent G4112A whole genome microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Marie-Therese Fischer

PROVIDER: E-GEOD-32915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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