ENCODE Cold Spring Harbor Labs Long RNA-seq (hg18)

ABSTRACT: This track depicts high throughput sequencing of long RNAs (>200 nt) from RNA samples from tissues or subcellular compartments from ENCODE cell lines. The overall goal of the ENCODE project is to identify and characterize all functional elements in the sequence of the human genome. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Cells were grown according to the approved ENCODE cell culture protocols. Sample preparation and sequencing: K562 and GM12878 total cell, total RNA: Standard Illumina Pair-end kit with the sole exception that a "tagged" random hexamer was used to prime the 1st strand synthesis: 5'-ACTGTAGGN6-3'. The addition of this tag is what permits

ORGANISM(S): Homo sapiens

SUBMITTER: UCSC ENCODE DCC 

PROVIDER: E-GEOD-32931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress