Exon arrays from HeLa cells transfected with GFP-ZRANB2 vector

ABSTRACT: Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3M-bM-^@M-^Y ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7). It has been shown to alter splicing patterns of Tra2M-NM-21 minigene primary transcripts in a dose-dependent manner. It is still unclear what role ZRANB2 plays in the regulation of alternative splicing. To determine the endogenous transcripts that are targeted by ZRANB2 at the genome wide level, we decided to perform exon microarray studies and analyzed the suitability of this method to examine splicing differences in human cells overexpressing a splicing factor. GFP-ZRANB2 construct containi

ORGANISM(S): Homo sapiens

SUBMITTER: Prof.Brian Morris 

PROVIDER: E-GEOD-32932 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress