Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data in Arabidopsis fry1-6, xrn2-4, xrn3-3 and xrn4-6 mutants.

ABSTRACT: We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis fry1-6, xrn2-4, xrn3-3 and xrn4-6 mutants. The total RNA was prepared from 15-day-old Arabidopsis plants grown on the agar plates and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yukio Kurihara

PROVIDER: E-GEOD-32977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Surveillance of 3' Noncoding Transcripts Requires FIERY1 and XRN3 in Arabidopsis.

Kurihara Yukio Y Schmitz Robert J RJ Nery Joseph R JR Schultz Matthew D MD Okubo-Kurihara Emiko E Morosawa Taeko T Tanaka Maho M Toyoda Tetsuro T Seki Motoaki M Ecker Joseph R JR

G3 (Bethesda, Md.) 20120401 4

Eukaryotes possess several RNA surveillance mechanisms that prevent undesirable aberrant RNAs from accumulating. Arabidopsis XRN2, XRN3, and XRN4 are three orthologs of the yeast 5'-to-3' exoribonuclease, Rat1/Xrn2, that function in multiple RNA decay pathways. XRN activity is maintained by FIERY1 (FRY1), which converts the XRN inhibitor, adenosine 3', 5'-bisphosphate (PAP), into 5'AMP. To identify the roles of XRNs and FRY1 in suppression of non-coding RNAs, strand-specific genome-wide tiling a ...[more]

PMID: 22540040

Similar Datasets

Expression data in Arabidopsis axe1-5 and met1-3 mutants.

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis axe1-5 and met1-3 mutants. Fifteen-day-old Arabidopsis plants grown on the agar plates were harvested. The total RNA was prepared from 15-day-old Arabidopsis plants (axe1-5 (hda6 mutant), DR5 (parental line of axe1-5), met1-3 and Col) and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

2011-05-15 | E-GEOD-23950 | biostudies-arrayexpress

Expression data for the mom1-2 mutant of Arabidopsis

Project description:Heterochromatin is an inert region in the genome and composed of mainly remnants of transposons and repetitive elements. In Arabidopsis, the major heterchromatin regions are present at around centromeres (pericentromeric regions) and at a region on the short arm of chromosome 4 (heterochromatin knob). Histones and DNAs in heterochromatin have characteristic features with abundant H3H9me2 and cytosine methylation, respectively. Here, by using a genome tiling array, we showed that a subset of heterochromatin loci are silenced by the action of Morpheus' molecule 1 (MOM1) that is an epigeneic regulator for transcriptional gene silencing independent of global DNA and histone modification. Most of the up-regulated loci in the mom1 mutant carried sequences related to transposable elements but none of them was annotated as functional transposons. No specific subclass of transposons was targeted by MOM1 and loci that were unrelated to transposons but flanked by short tandem repeats were also shown to be under the control of MOM1. The results suggest the presence of an unknown level of regulatory network maintaining the silent state of heterochromain in the genome. Keywords: Epigenetic regulation of endogenous loci by Morpheus' Molecule 1 (MOM1) Three-week-old Arabidopsis plants (Col-0 and mom1-2) grown on soil were subjected to RNA extraction and the total RNA samples were used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

2010-09-26 | E-GEOD-13092 | biostudies-arrayexpress

Expression data in Arabidopsis aba2-1 mutant, cyp707a1-1a2-1a3-1 triple mutant seeds, embryo and endosperm tissues

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in aba2-1, cyp707a1-1a2-1a3-1 triple mutants seeds, embryo and endosperm tissues Freshly harvested seeds were imbibed in 8.5 cm Petri dishes containing two filter papers (approximately 7 cm diameter) and 2 mL of water and harvested. Dissection of embryo and endosperm from 24-h-imbibed seeds was carried out under a stereoscopic microscope. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

2010-04-15 | E-GEOD-15700 | biostudies-arrayexpress

Expression data in Arabidopsis wild type, ahg2-1 and ahg2-1ags1-1 mutants

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis wild type, ahg2-1 and ahg2-1ags1-1 mutants. The total RNA was prepared from 15-day-old Arabidopsis plants grown on the agar plates and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

2013-08-09 | E-GEOD-46128 | biostudies-arrayexpress

Expression data in Arabidopsis hda6 mutant axe1-5 and its parental line DR5 under cold and control conditions.

Project description:To understand the role of Arabidopsis histone deacetylase HDA6 in plant cold acclimation, we have employed transcriptional profiling of the hda6 mutant and its parental line under cold and control conditions to identify genes differentially expressed in the hda6 mutant under cold and control conditions. Aligent’s Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K) were used. Arabidopsis hda6 mutant axe1-5 and its parental line DR5 were grown in MS agar plates for 2 weeks (16 hours light / 8 hours dark). For cold treated sample, the plants were subjected for cold treatment at 2?C for 3 days (12 hours light / 12 hours dark). Then total RNA was prepared and used for the microarray hybridization. Three replicative hybridization experiments for each array were carried out using the independent biological samples.

2011-01-27 | E-GEOD-26873 | biostudies-arrayexpress

Expression data for drought-, cold, high-salinity and ABA treatment in Arabidopsis plants

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome under drought, cold, high-salinity and ABA treatment conditions and idenfied many stress- or ABA- responsive putative functional RNAs and fully-overlapping sense-antisense transcripts in Arabidopsis genome. Keywords: stress response Two-week-old Arabidopsis plants grown on the agar plates were subjected to the stress- or ABA- treatments. The total RNA was prepared from the treated- and untreated- plants, and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

2008-11-19 | E-GEOD-9646 | biostudies-arrayexpress

Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.

2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress

Arabidopsis thaliana response to ozone

Project description:Comparison of Arabidopsis seedlings exposed to 500 ppb ozone with air treated control plants.

2005-05-25 | E-MEXP-342 | biostudies-arrayexpress

Difference in gene expression between WT (Col-0) and atxrn4-3 mutant under non-stress and heat stress condition

Project description:Arabidopsis 5’-3’ exoribonuclease, AtXRN4, a homolog of yeast Xrn1p, functions in degradation of uncapped RNAs after de-capping step. While Xrn1p-dependent on plant XRN4’s targets for degradation is still limited. For understanding biological function of AtXRN4, we tested survivability of atxrn4 mutants under heat stress. Our results showed that atxrn4 mutants increased survival rate under short-term degradation is a main mRNA decay in yeast, knowledge heat stress compared with WT plants. Our microarray and mRNA decay assay showed that loss of AtXRN4 function caused reduction of mRNA degradation of heat shock factor A2 (HSFA2) and ethylene response factor 1 (ERF1). HSFA2 has been known as a key regulator in heat acclimation, was found as a target for AtXRN4 for degradation at non-stress condition. Heat stress applied on atxrn4-3 hsfa2 double mutant severely lacked heat tolerance phenotype of atxrn4 mutant. These results suggest that AtXRN4-mediated mRNA degradation linked to suppress heat acclimation. In the study here, 2 week-old WT and atxrn4-3 mutant plants were exposure to non-stress (22oC) and heat-stress (37oC, 1 h). Custom microarray was applied to acquire expression profile of 32788 Arabidopsis genes. 3 biological repeats of WT (non-stress), WT(heat stress), atxrn4-3 (non-stress) and atxrn4-3 (heat stress) were used for microarray analysis

2015-07-14 | E-GEOD-66369 | biostudies-arrayexpress

Gene expression profiles in Arabidopsis plants during the treatment of AgNO3.

Project description:Camalexin, an indolic secondary metabolite, is the magor phytoalexin produced by Arabidopsis thaliana. Camalexin biosynthesis is induced by abiotic stresses such as heavy metal treatment (e.g AgNO3). With the aim of identifying positive regulators of camalexin biosynthesis in Arabidopsis, we listed putative transcription factor genes strongly induced during the time course of AgNO3 treatment from a transcriptome analysis. AgNO3 induced gene expression in Arabidopsis was measured at 48 and 72 hours after the 0.1 mM AgNO3 treatment. Arabidopsis seedlings were germinated under sterile conditions on GM-agar plates. Seedlings (one-week after germination) were then transferred to GM liquid culture and shaken for two weeks. Plants were then treated with 0.1 mM AgNO3 and sampled at 0 h, 48 h and 72 h after the treatment. Three independent experiments were performed at each time (0, 48, or 72 h) using different plants for each experiment.

2011-12-22 | E-GEOD-34606 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data