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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Activated YAP1 stimulates cardiomyocyte proliferation

ABSTRACT: Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later. 2 group comparison, n=4 biological replicates per group

ORGANISM(S): Rattus norvegicus

SUBMITTER: William Pu

PROVIDER: E-GEOD-33019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Activated YAP1 stimulates cardiomyocyte proliferation

Project description:Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later.

2012-01-12 | GSE33019 | GEO

Regulation of gene expression by YAP1

Project description:In order to define YAP1-specific gene expression patterns in gastric cancer, the constitutively active mutant YAP1 (YAP1-S127A) was over-expressed in MKN45 gastric cancer cells. Defined gene expression signature was later used to stratify gastric cancer patients according to the presence of the YAP1-activated signature. Three groups of samples are included: 1. Mock control; 2. Vector control; 3. YAP-S127A expression. Gene expression profiles of YAP-S127A mutant-expressing cells were compared to that of mock and vector control. Experiments were done in MKN45 gastric cancer cells.

2013-10-01 | E-GEOD-41387 | biostudies-arrayexpress

Regulation of gene expression by YAP1

2013-10-01 | GSE41387 | GEO

RASSF1A promotes its tumor-suppressive function via YAP1 target genes

Project description:The aim of the study was to determine the effect of the tumour suppressor RASSF1A in a stably induced YAP system, as both are members of the growth regulatory HIPPO pathway. YAP1 expression was induced by Doxycycline in stably transfected HEK293 cells. In addition the cells were transfected with RASSF1A/GFP or GFP. Transfected cells were FACS-sorted by the GFP-signal and lysed for transcriptome analysis.

2017-05-15 | E-MTAB-4781 | biostudies-arrayexpress

AP-MS_XL experiment to characterize YAP1/LATS/PTPN14 complex

Project description:In this project we investigated the topological composition of ternary complex PTPN14/LATS1/PTPN14. For this purpose, we performed affinity purification with Strep tag of Strep-HA YAP1 and subsequent XL reaction.

2023-03-04 | PXD039440 | Pride

Role of Yes-Associated Protein 1 (YAP1) in rhabdomyosarcoma

Project description:Doxycycline-inducible YAP1 S127A-driven rhabdomyosarcoma (RMS) tumors, control skeletal muscle and regressed tumors following YAP1 normalization by doxycycline withdrawal were compared to determine the YAP1-regulated gene expression profile relevant to RMS formation. To characterize the role of YAP1 in embryonal RMS at the molecular level and identify a gene signature for YAP1 activity readout, we compared the gene expression profiles of our YAP1-driven ERMS with control donor skeletal muscle (SKM) and doxycycline-withdrawn regressing tumors by microarray (doxycycline withdrawal for 3 or 6 days; OFF3 and OFF6, respectively). We next extracted a list of genes regulated by YAP1 in our YAP1-driven ERMS tumors (TUM) versus the 3 other conditions: skeletal muscle control (SKM), Doxycycline-withdrawn 3 days (OFF3) and 6 days (OFF6). The overlap between the 3 lists identified a subset of 633 common upregulated genes, named the YAP1-ERMS_UP signature, as well as 249 common downregulated genes, termed the YAP1-ERMS_DOWN signature. Proliferative pathways and transcriptional targets of E2F factors were highlighted in the YAP1-ERMS_UP genes, while muscle differentiation and trancriptional targets of myogenic factors Myod1 and Mef2 were highlighted in the YAP1-ERMS_DOWN genes. Tumor regression conditions (OFF3 days; OFF6 days) as well as control muscle (CTL) were compared with tumors at day 0 of doxycycline withdrawal (TUM). 3 samples for each conditions were used.

2014-08-12 | E-GEOD-47198 | biostudies-arrayexpress

AP-MS of Strep-HA YAP1 mutants (S61A, S109A, S127A, S138A, S143A, S367A, 5SA)

Project description:In this project we investigated the effect of YAP1 mutations on the interactome. We investigate the effect of S61A, S109A, S127A, S138A, S143A, S367A, 5SA.

2023-03-04 | PXD039445 | Pride

human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

Project description:Transcriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA. Two-condition experiment, Control siRNA vs.YAP1 siRNA hTERT-RPE1 cell spheroids. Biological replicates: 1 Control siRNA, 1YAP1 siRNA transfected, independently grown and harvested. Bothreplicates per array.

2015-02-01 | E-GEOD-54146 | biostudies-arrayexpress

Role of Yes-Associated Protein 1 (YAP1) in rhabdomyosarcoma

2014-08-12 | GSE47198 | GEO

AP-MS of Strep-HA YAP1 mutants (S61A, S109A, S127A, S138A, S143A, S367A, 5SA, delta WW1, delta WW2, delta WW1/WW2)

Project description:In this project we investigated the effect of YAP1 mutations on the interactome. We investigate the effect of S61A, S109A, S127A, S138A, S143A, S367A, 5SA, delta WW1, delta WW2, delta WW1/WW2

2023-03-04 | PXD039443 | Pride

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