Transcription profiling of human fetal lung epithelial cells in undifferentiated state and after type II cell differentiation induced by DCI treatment

ABSTRACT: Studies of human fetal lung in explant culture and in isolated epithelial cells have demonstrated that both glucocorticoids and cyclic AMP promote differentiated alveolar type II cell phenotype as assessed by ultrastructural morphology and surfactant production. This project profiles changes in gene expression associated with hormone induced differentiation. Undifferentiated human fetal lung (13-20 wk) epithelial cells were cultured in serum-free medium (control) or with dexamethasone/8-Bromo cyclic AMP/isobutylmethylxanthine (DCI) to promote type II cell differentiation. RNA from five sets of experiments (10 samples) was evaluated using the U133A Affymetrix GeneChip set. Experiment Overall Design: Cells were isolated from 13 individual lungs and the cells from each were cultured in th

ORGANISM(S): Homo sapiens

SUBMITTER: Philip Ballard 

PROVIDER: E-GEOD-3306 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress