Transcription profiling of human HNF1beta mutated vs wildtype embryonic kidney cell line HEK293 to identify potential HNF1beta target genes whose activity may be deregulated in human patients

ABSTRACT: Hepatocyte nuclear factor 1beta (HNF1beta, TCF2) is a tissue-specific transcription factor whose mutation in humans leads to renal cysts, genital malformations, pancreas atrophy and maturity onset diabetes of the young (MODY5). Furthermore, HNF1beta overexpression has been observed in clear cell cancer of the ovary. To identify potential HNF1beta target genes whose activity may be deregulated in human patients we established a human embryonic kidney cell line (HEK293) expressing HNF1beta conditionally. Using Flp recombinase we introduced wildtype or mutated HNF1beta at a defined chromosomal position allowing a most reproducible induction of the HNF1beta derivatives upon tetracycline addition. By oligonucleotide microarrays we identified 25 HNF1beta regulated genes. By an identical approach

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-3308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress