Breast cancer and liver tissue biopsies used to develop and validate an index for liver contamination in metastatic breast cancer

ABSTRACT: PURPOSE: To develop an index capable of detecting and quantifying the extent of liver RNA contamination in liver biopsies of metastatic breast cancer METHODS: We developed a microarray-based gene expression liver index by comparing the expression levels of genes in liver tissue biopsies and in primary breast cancer biopsies. The predictive performance of the index was then evaluated in defined mixtures of liver and breast cancer RNA. A calibration curve was established to allow estimation of the liver RNA content in unknown metastatic breast cancer samples. A set of liver biopsies and primary breast cancer biopsies were used to identify genes that were expressed significantly higher in liver tissue. These genes were used in a liver index, which was validated in a new set of liver and pr

ORGANISM(S): Homo sapiens

SUBMITTER: Christos Hatzis 

PROVIDER: E-GEOD-33116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress