Transcription profiling of human small airway bronchial epithelium to assess gene expression in phenotypically normal smokers compared to matched non-smokers

ABSTRACT: The earliest morphologic evidence of changes in the airways associated with chronic cigarette smoking is in the small airways. To help understand how smoking modifies small airway structure and function, we developed a strategy using fiberoptic bronchoscopy and brushing to sample the human small airway (10th-12th order) bronchial epithelium to assess gene expression (Affymetrix HG-U133A array) in phenotypically normal smokers (n=6, 24 ± 4 pack-yr) compared to matched non-smokers (n=5). Compared to samples from the large (2nd to 3rd order) bronchi, the small airway samples had a higher proportion of ciliated cells, but less basal, undifferentiated, and secretory cells. The small, but not large, airway samples included Clara cells, a cell found only in the small airway epithelium, and the sm

ORGANISM(S): Homo sapiens

SUBMITTER: Adriana Heguy 

PROVIDER: E-GEOD-3320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress