RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryo

ABSTRACT: Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient. Most SCNT-generated embryos die after implantation because of unidentified, complex epigenetic errors in the process of postimplantation embryonic development. Here, we identified the most upstream level of dysfunction leading to impaired development of clones by using RNA interference (RNAi) against Xist, a gene responsible for X chromosome inactivation (XCI). A prior injection of Xist-specific short interfering (si)RNA into reconstructed oocytes efficiently corrected the SCNT-specific aberrant Xist expression at the morula stage, but failed to do so thereafter at the blastocyst stage. However, we found that shortly after implantation this aberrant XCI status in cloned embryos had been corrected autonomously i

ORGANISM(S): Mus musculus

SUBMITTER: Kimiko Inoue 

PROVIDER: E-GEOD-33208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress