Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Differential Expression of Novel Potential Regulators in Hematopoietic Stem Cells.

ABSTRACT: Pairwise comparisons of purified mouse LT-HSC, ST-HSC and MPP. The hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic subpopulations in adult mouse bone marrow: long-term hematopoietic stem cells (HSC), short-term HSC, and multipotent progenitors. All three populations are capable of differentiating into a spectrum of mature blood cells, but differ in their self-renewal and proliferative capacity. We identified numerous novel potential regulators of HSC self-renewal and proliferation that were differentially expressed between these closely related cell populations. Many of the differentially expressed transcripts fit into pathways and protein complexes not previously identified in HSC, providing evidence for new HSC regulatory units. Extending these observations to the protein level, we demonstrate expression of several of the corresponding proteins, which provide novel surface markers for HSC. We discuss the implications of our findings for HSC biology. In particular, our data suggest that cell-cell and cell-matrix interactions are major regulators of long-term HSC, and that HSC themselves play important roles in regulating their immediate microenvironment. An experiment design type where the label orientations are reversed. exact synonym:flip dye, dye flip Keywords: dye_swap_design Computed

ORGANISM(S): Mus musculus

SUBMITTER: Susan Prohaska

PROVIDER: E-GEOD-3321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular signatures of quiescent, mobilized and leukemia-initiating hematopoietic stem cells.

Forsberg E Camilla EC Passegué Emmanuelle E Prohaska Susan S SS Wagers Amy J AJ Koeva Martina M Stuart Joshua M JM Weissman Irving L IL

PloS one 20100120 1

Hematopoietic stem cells (HSC) are rare, multipotent cells capable of generating all specialized cells of the blood system. Appropriate regulation of HSC quiescence is thought to be crucial to maintain their lifelong function; however, the molecular pathways controlling stem cell quiescence remain poorly characterized. Likewise, the molecular events driving leukemogenesis remain elusive. In this study, we compare the gene expression profiles of steady-state bone marrow HSC to non-self-renewing m ...[more]

PMID: 20098702

Publication: 1/2

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Dataset Information

Differential Expression of Novel Potential Regulators in Hematopoietic Stem Cells.

Publications

Molecular signatures of quiescent, mobilized and leukemia-initiating hematopoietic stem cells.

Similar Datasets

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