Transcriptome of rice seedling and callus based on RNA-seq method

ABSTRACT: To support our research of epigenomics in rice genome, we conducted massively parallel pyrosequencing of mRNAs (RNA-seq) using rice seedling and callus tissues. We obtained a total of 40.1 M reads from seedling and 29.6 M reads from callus.The RNA-seq data derived from the replicates showed high correlations (Spearman’s rank correlation coefficient, SC, 0.96 for seedling, 0.88 for callus). Our RNAseq data derived from the seedling also showed a high correlation with the recently published rice RNA-seq data that was also from two-week old seeding tissue (Lu et al., 2010, PMID: 20627892) (SC=0.87). Two biological replicates of each tissue was applied in RNA-seq. Each biological replicate was sequenced twice in separate lane.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Yufeng Wu 

PROVIDER: E-GEOD-33265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress