Response of Bacillus halodurans C-125 wild-type and OLE-OAP knockout cells to acute ethanol stress

ABSTRACT: OLE RNA (Ornate, Large, Extremophilic RNA) is a large, noncoding RNA from extremophilic and/or anaerobic Firmicutes that binds to an integral membrane protein (OAP, OLE-Associated Protein) and localizes to the cell membrane. It is highly abundant in cells, and knockouts show increased sensitivity to short-chain alcohols, especially ethanol. We treated Bacillus halodurans C-125 cells (both wild-type and OLE-OAP knockouts) with 5% (v/v) ethanol for 3 hours to determine differences in their response to this stress. There are almost no significant differences between cells before ethanol stress, while after ethanol stress a small number (~40) genes show differences in expression between the two strains. These genes show little connection between their function, however, leading us to conclude

ORGANISM(S): Bacillus halodurans

SUBMITTER: Jason Wallace 

PROVIDER: E-GEOD-33303 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress