Gene expression changes in breast epithelial cells surviving treatment with TRAIL (TNF-Related Apoptosis Inducing Ligand)

ABSTRACT: In this study, we subjected the phenomenon of fractional killing in a clonal population of TRAIL-sensitive cells to careful analysis. We asked whether cells that survive initial exposure to TRAIL are sensitive or resistant to subsequent treatment with TRAIL or other apoptosis-inducing agents. We observed that TRAIL survivor cells were highly resistant to a subsequent treatment with TRAIL 24hr later but that this resistance disappeared following several days in culture. Resistance did not involve downregulation of TRAIL receptors and extended to death ligands that bind different classes of receptors: TRAIL survivors were transiently resistant to FasL and vice versa. Gene expression analysis revealed that NF-kB-mediated inflammatory genes were activated in transiently resistant survivor ce

ORGANISM(S): Homo sapiens

SUBMITTER: Deborah Flusberg 

PROVIDER: E-GEOD-33340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress