Expression profiling of severe growth abortion (SGA) in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

ABSTRACT: We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in SGA lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in crown tissues of SGA line. On the other hand, photosynthesis-related genes were highly down-regulated in crown tissues of SGA line. In addition, transcript accumulation levels of shoot apical meristem (SGA)-related genes such as knotted-1 like homeobox (Knox) genes were also repressed in SGA line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 23 selected genes were performed in total. Of the examined genes, 20 genes

ORGANISM(S): Aegilops tauschii x Triticum turgidum

SUBMITTER: Shigeo Takumi 

PROVIDER: E-GEOD-33357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress