Transcription profiling of murine dendritic cells in the presence and absence of interferon (IFN)-gamma to identify a tolerogenic signature in IDO-competent dendritic cells.

ABSTRACT: Although much is known on the transcriptional profiles of dendritic cells (DCs) during maturation, the molecular switches critical for the acquisition of a tolerogenic program by DCs are still obscure. In the present study, we explored the gene expression pattern of CD8+ DCs purified from the mouse spleen and treated with interferon (IFN)-gamma. The cytokine, indeed, potentiates the tolerogenic potential of this DC subset via induction of the immunosuppressive tryptophan catabolism mediated by indoleamine 2,3-dioxygenase (IDO). By comparing the expression of the IFN-gamma-modulated genes in IDO+ versus IDO- murine DCs, we found a consistent and selective association of the IDO-competent phenotype with the down-modulation of the Tyrobp gene, encoding the adapter molecule DAP12. IFN-gamma-me

ORGANISM(S): Mus musculus

SUBMITTER: Silvio Bicciato 

PROVIDER: E-GEOD-3337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress