Identification of osteosarcoma driver genes by integrative analysis of copy number and gene expression data

ABSTRACT: High-grade osteosarcoma is a tumor with a complex genomic profile, occurring primarily in adolescents with a second peak at middle age. The extensive genomic alterations obscure the identification of genes driving tumorigenesis during osteosarcoma development. In order to identify such driver genes, we integrated DNA copy number profiles (Affymetrix SNP 6.0) of 32 diagnostic biopsies with 84 expression profiles (Illumina Human-6 v2.0) of high-grade osteosarcoma as compared with its putative progenitor cells, i.e. mesenchymal stem cells (n=12) or osteoblasts (n=3). In addition, we performed paired analyses between copy number and expression profiles of a subset of 29 patients for which both DNA and mRNA profiles were available. Integrative analyses were performed in Nexus Copy Number softwa

ORGANISM(S): Homo sapiens

SUBMITTER: Marieke Kuijjer 

PROVIDER: E-GEOD-33383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress