Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human MAIT and CD8++ cell development

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE33374: Expression data from healthy human CD161++CD8aa and CD161++CD8ab T cells GSE33424: Expression data from human cord blood CD161++/CD161+/CD161- CD8+ T cell subsets Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Natasha Sahgal

PROVIDER: E-GEOD-33425 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Human MAIT and CD8αα cells develop from a pool of type-17 precommitted CD8+ T cells.

Walker Lucy J LJ Kang Yu-Hoi YH Smith Matthew O MO Tharmalingham Hannah H Ramamurthy Narayan N Fleming Vicki M VM Sahgal Natasha N Leslie Alistair A Oo Ye Y Geremia Alessandra A Scriba Thomas J TJ Hanekom Willem A WA Lauer Georg M GM Lantz Olivier O Adams David H DH Powrie Fiona F Barnes Eleanor E Klenerman Paul P

Blood 20111115 2

Human mucosal associated invariant T (MAIT) CD8(+) and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 ((++)) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161(++)CD8αβ(+) T cells exist in cord blood, from which a prominent MAIT cell (TCR Vα7.2(+)) population emerges post-natally. During this expa ...[more]

PMID: 22086415

Similar Datasets

Expression data from healthy human CD161++CD8aa and CD161++CD8ab T cells

Project description:We used microarrays to compare gene expression between healthy human CD161++CD8aa and CD161++CD8ab T cells. We sorted freshly obtained peripheral blood mononucleocytes from healthy controls by first using magnetic beads to negatively select for CD8a+ T cells, fluorescently labelling isolated cells with antibodies and subsequent sorting using MoFlow MLS cell sorter (DAKO). Sorted samples were snap frozen. RNA was later extracted from the sorted populations prior to hybridization to Affymetrix arrays.

2012-01-25 | E-GEOD-33374 | biostudies-arrayexpress

Expression data from human cord blood CD161++/CD161+/CD161- CD8+ T cell subsets

Project description:We used microarray to compare gene expression between CD161++/CD161+/CD161-CD8+ T cells from human cord blood. Lymphocytes from freshly obtained human cord blood samples were isolated by Ficoll density centrifugation. CD8+ T cells were purified by negative selection using magnetic beads and subsequently labelled with fluorescent antibodies prior to sorting using MoFlo MLS cell sorter (Dako).

2012-01-25 | E-GEOD-33424 | biostudies-arrayexpress

Expression data from healthy human CD161++CD8aa and CD161++CD8ab T cells

Project description:We used microarrays to compare gene expression between healthy human CD161++CD8aa and CD161++CD8ab T cells.

2012-01-25 | GSE33374 | GEO

Murine PDAC cell lines

Project description:Expression profiles of 28 murine pancreatic cancer cell lines isolated from a KrasG12D-based mouse model of pancreatic cancer mRNA of 28 low passaged murine pancreatic cancer cell lines was extracted and hybridized to Affymetrix microarrays

2012-09-06 | E-GEOD-40609 | biostudies-arrayexpress

Gene expression profiling of Type II Enteropathy-associated T-cell lymphoma

Project description:Type II Enteropathy-associated T-cell lymphoma (Type II EATL) is an aggressive intestinal T-cell lymphoma with poor prognosis and has not been molecularly profiled. Through targeted amplicon sequencing, we identified a large portion of Type II EATL samples that harbor mutations in the STAT5B, JAK3 and GNAI2 genes. Here we performed gene expression profiling on four Type II EATL samples in order to better characterize this disease. As Type II EATL is suggested to arise from CD8+ IELs, we integrated our data with publicly available profile of CD8Î±Î± and CD8Î±Î² T-cells from healthy donors (GSE33374). Gene expression profiling independently demonstrated strong enrichment of several aspects of GPCR and JAK-STAT signaling pathways. Moreover, an significant association was identified with genes containing STAT5B binding sites in their promoters. Microarray gene expression profiling was performed on 4 Type II Enteropathy-associated T-cell lymphoma and a combination of unsupervised and supervised clustering was performed to determine any differentially activated pathways between samples with tumor and healthy human CD161++CD8aa and CD161++CD8ab T cells (found under accession number GSE33374)

2016-03-03 | E-GEOD-70652 | biostudies-arrayexpress

Homo sapiens

Project description:Expression data from healthy human CD161++CD8aa and CD161++CD8ab T cells

| PRJNA154247 | ENA

Global expression profiling of peripheral Qa-1 class Ib-restricted CD8aa+TCRab+ regulatory T Cells reveals innate-like features: implications for immune regulatory repertoire

Project description:To better understand the function of CD8aa Tregs, we have recently characterized several CD8aa+TCRab+ T cell clones and lines that are physiologically primed and are involved in recovery and protection from EAE (REF). In this report, we present a comparison of global gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells. The results of microarray data analysis are confirmed by real-time PCR and flow cytometry for better accuracy and phenotype expression. Our study reveals a unique gene signature in the CD8aa Tregs, including enhanced expression of several inhibitory molecules associated with the maintenance of peripheral T cell homeostasis. The identification of differentially expressed molecules by CD8aa Tregs should facilitate future functional studies in this area. gene expression patterns in CD8aa Tregs versus OT-1 CD8aa+TCRab+ T cells

2011-08-31 | E-GEOD-22985 | biostudies-arrayexpress

Expression data from lungs of rats with pulmonary hypertension

Project description:Pulmonary hypertension is a frequent consequence of left heart disease and congestive heart failure (CHF) and causes extensive lung vascular remodelling which leads to right ventricular failure. Functional genomics underlying this structural remodelling are unknown but present potential targets for novel therapeutic strategies. We used microarrays to detail the gene expression underlying vascular remodeling in the pathogenesis of pulmonary hypertension and identified distinct classes of up-regulated genes during this process. Control rat lung samples were compared to samples of aortic banding rat lungs which exhibit pulmonary hypertension

2009-06-16 | E-GEOD-16624 | biostudies-arrayexpress

Changes in gene expression caused by loss of Med1 in the mouse prostate

Project description:MED1 is a transcriptional coactivator for gene-specific activators involved in growth and development, we were interested in identifying MED1 target genes potentially involved in prostate development by cDNA microarray. Med1 was conditional knocked out in mice prostate. We performed cDNA microarray with two sets: (1) RNA isolated from three WT ventral prostates and three MT ventral prostates; (2) RNA isolated from three WT lateral prostates and three MT lateral prostates. We used microarrays to detail the global programme of gene expression underlying how Med1 was involved in mice prostate development by regulating targeted genes expression 12 mice prostate samples were divided into two groups: (1) three WT ventral prostates and three MT ventral prostates; (2) three WT lateral prostates and three MT lateral prostates.

2015-05-22 | E-GEOD-69124 | biostudies-arrayexpress

Hypothalamic expression differences between hypertensive BPH/2J and normotensive BPN/3J mouse strains

Project description:Identification of hypothalamic genes whose expression differs between high blood pressure (BPH/2J) and normal blood pressure (BPN/3J) Schlager mouse strains at age 6 weeks (young) and 26 weeks (mature) using Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays. The whole hypothalamus was removed from young (6-week-old) and adult (26-week-old) BPH/2J hypertensive mice and age-matched normotensive BPN/2J mice (n=6/group) at peak of 24 h blood pressure. No pooling was performed. After extraction of RNA, cRNA was prepared and arrays performed using Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays at the Ramaciotti Gene Function Analysis facility, University of New South Wales in Sydney, Australia.

2011-04-11 | E-GEOD-25076 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data