MiR-124 acts through coREST to control the onset of Sema3A sensitivity in navigating retinal growth cones

ABSTRACT: During axon pathfinding, growth cones commonly exhibit changes in sensitivity to guidance cues that follow a strict timetable, even in the absence of pathway feedback, implicating cell-intrinsic regulation. Cellular timer mechanisms, however, are poorly understood. Here we have investigated microRNAs in the timing control of Sema3A sensitivity in retinal ganglion cell (RGC) growth cones. A developmental profiling screen identified miR-124 as a candidate timer. Loss of miR-124 delayed the onset of Sema3A sensitivity and concomitant Neuropilin-1 (NRP-1) receptor expression, and caused cell autonomous pathfinding errors. CoREST, a cofactor of a NRP1 repressor, was identified as a novel target and mediator of miR-124 for this highly specific temporal aspect of RGC growth cone responsiveness. O

ORGANISM(S): Xenopus laevis

SUBMITTER: Cei Abreu-Goodger 

PROVIDER: E-GEOD-33444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress