Extracellular vesicles from neural stem cells transfer IFN-? via Ifngr1 to activate Stat1 signalling in target cells

ABSTRACT: The idea that stem cell therapies work only via cell replacement is challenged by the observation of consistent intercellular molecule exchange between the graft and the host. Here we defined a mechanism of cellular signaling by which neural stem/precursor cells (NPCs) communicate with the microenvironment via extracellular vesicles (EVs), and we elucidated its molecular signature and function. We observed cytokine-regulated pathways that sort proteins and mRNAs into EVs. We described induction of interferon gamma (IFN-?) pathway in NPCs exposed to proinflammatory cytokines that is mirrored in EVs. We showed that IFN-? bound to EVs through Ifngr1 activates Stat1 in target cells. Finally, we demonstrated that endogenous Stat1 and Ifngr1 in target cells are indispensable to sustain the activ

ORGANISM(S): Mus musculus

SUBMITTER: Stefano Pluchino 

PROVIDER: E-GEOD-33527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress