Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

R. sphaeroides Δirr vs. R. sphaeroides 2.4.1 under microaerobic conditions

ABSTRACT: Transcriptional profiling of R. sphaeroides Δirr compared to control R. sphaeroides 2.4.1 under microaerobic conditions. Two strain experiment under microarobic conditions (30 µM). 6 Biological replicas, independetly grown and harvested at OD660=0,4; 1-3 pooled in replicate 1, 4-6 pooled in replicate 2

ORGANISM(S): Rhodobacter sphaeroides 2.4.1

SUBMITTER: Verena Peuser

PROVIDER: E-GEOD-33534 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides Δirr under iron limitation (-Fe) compared to control R. sphaeroides Δirr under normal growth conditions (+Fe). Two strain experiment under normal iron (+Fe) and iron limitation (-Fe) conditions. 6 Biological replicates, independently grown and harvested at OD660=0,4; 1-3 pooled in replicate 1, 4-6 pooled in replicate 2

2011-11-09 | E-GEOD-33533 | biostudies-arrayexpress

R. sphaeroides Δlov vs. R. sphaeroides 2.4.1 (singlet oxygen stress, aerobic conditions)

Project description:Transcriptional profiling of R. sphaeroides Δlov compared to control R. sphaeroides 2.4.1 under singlet oxygen stress, aerobic conditions Two-strain experiment with overnight cultures of the wild type 2.4.1 and the Δlov mutant. Both were diluted to an OD660 of 0.2 and gassed to adjust aerobic conditions (≈ 170 µM O2). 0.2 µM of methylene blue was added to the cultures to act as photosensitizer. After one doubling time of growth in the darkness cells were illuminated with 800 W m-2 of white light for 7 min.

2011-10-25 | E-GEOD-33260 | biostudies-arrayexpress

Transcriptional profiling of R. sphaeroides Δirr

Project description:This SuperSeries is composed of the following subset Series: GSE33533: R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions GSE33534: R. sphaeroides Δirr vs. R. sphaeroides 2.4.1 under microaerobic conditions Refer to individual Series

2011-11-09 | E-GEOD-33535 | biostudies-arrayexpress

R. sphaeroides Δlov vs. R. sphaeroides 2.4.1 (blue light, semiaerobic conditions)

Project description:Transcriptional profiling of R. sphaeroides Δlov compared to control R. sphaeroides 2.4.1 under blue light, semiaerobic conditions. Two-strain experiment under blue light illumination (20µmol m-2 s-1) and semiaerobic (90 µM O2) conditions

2011-10-25 | E-GEOD-33259 | biostudies-arrayexpress

R. sphaeroides delta-cryB vs. R. sphaeroides 2.4.1 under blue light, semiaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides delta-cryB compared to control R. sphaeroides 2.4.1 under blue light, semiaerobic conditions. Two-strain experiment under blue light illumination (20µmol m-2 s-1) and semiaerobic (90 µM) conditions

2011-11-09 | E-GEOD-33555 | biostudies-arrayexpress

R. sphaeroides delta-cryB vs. R. sphaeroides 2.4.1 photo-oxidative stress, aerobic conditions

Project description:Transcriptional profiling of R. sphaeroides delta-cryB compared to control R. sphaeroides 2.4.1 under photo-oxidative stress, aerobic conditions. Two-strain experiment under 20' photo-oxidative stress (singlet oxygen generated by methylene blue under high light illumination) and aerobic (180 µM) conditions

2011-11-09 | E-GEOD-33553 | biostudies-arrayexpress

Rhodobacter sphaeroides WT 2.4.1 under singlet oxygen stress

Project description:Transcriptional and translational profiling of R. sphaeroides WT 2.4.1 under singlet oxygen stress compared to control (no stress) conditions RNA samples collected at time-point zero and at different time-points after singlet oxygen stress were analyzed by two-color microarrays

2012-11-14 | E-GEOD-42244 | biostudies-arrayexpress

Rhodobacter sphaeroides 2.4.1 pSurS versus pBBR under singlet oxygen stress.

Project description:To learn more about the function of SurS, we constructed an over-expression strain. For this purpose, the surS gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced SurS over-expression compared to an empty vector control. Total RNA from the over-expression (pSurS) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SurS-induced changes on transcriptome level. RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.

2014-07-04 | E-GEOD-59046 | biostudies-arrayexpress

R. sphaeroides pRPcrZ vs. R. sphaeroides pR4352 under low oxygen conditions

Project description:Transcriptional profiling of R. sphaeroides pRPcrZ compared to control R. sphaeroides pR4352 under low oxygen conditions. In this study we compared the transcriptome of a PcrZ over-expression strain to a control strain. PcrZ is the first small non-coding RNA, which is involved in the complex regulatory network of photosynthesis gene regulation in Rhodobacter sphaeroides. It counteracts the formation of photosynthetic complexes in a redox dependent manner. Two-strain experiment at low oxygen conditions (0.5 mg/L O2)

2012-08-14 | E-GEOD-37381 | biostudies-arrayexpress

R. sphaeroides Δlov vs. R. sphaeroides 2.4.1 (microarobic conditions)

Project description:Transcriptional profiling of R. sphaeroides Δlov comparing to control R. sphaeroides 2.4.1 under microarobic conditions Two strain experiment under microarobic conditions. 6 Biological replicas, independetly grown and harvested at OD660=0,8 - 1-3 pooled in replicate 1 and 4-6 pooled in replicate 2

2011-10-25 | E-GEOD-33194 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data